Antibody Panels and Kits

Combinations of antibody-related products that may include sets of antibodies in pairs and cocktails, control particles, buffers, and other kits and panels; for use in specific applications such as immunoassays and T-cell activation.

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MilliporeSigma™ Upstate™ Monomethyl-Histone H3 (Lys27), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C from date of receipt
Host Species	Rabbit
Applications	ChIP Assay,Electromobility Shift Assay,Flow Cytometry,Immunofluorescence,Immunohistochemistry,Western Blot
Form	Serum
Gene Accession No.	Q66I33
Includes	This ChIPAb+ Monomethyl-Histone H3 (Lys27) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Monomethyl-Histone H3 (Lys27)
Regulatory Status	RUO
Gene Symbols	H3F3A, H3F3B, H3F3
Gene ID (Entrez)	NM_003493
Formulation	Anti-monomethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide. ChIP Primers, β-Globin. One vial containing 75μL of 5μM of each primer specific for for human β-globin promoter. FOR: AGG ACA GGT ACG GCT GTC ATC REV: TTT ATG CCC AGC CCT GGC TC
Immunogen	The monomethyl-histone H3 (Lys27) antiserum is made against a synthetic 2X-branched peptide containing the sequence…ARmeKSA…in which meK corresponds to monomethyl-lysine 27 of human histone H3.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Monomethyl-Histone H3 (Lys27)

MilliporeSigma™ Upstate™ Estrogen Receptor αα, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Ascites
Gene Accession No.	P03372
Includes	This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Estrogen Receptor αα
Regulatory Status	RUO
Gene Symbols	ESR1, Era, ER-alpha, ESR, ER, NR3A1, ESRA, DKFZp686N23123
Gene ID (Entrez)	NM_000125.2
Formulation	Anti-ERα (mouse ascites). 1 vial containing 100mL ascites. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα. ChIP primers TFF1 (pS2). 1 vial containing 75mL of 5μM of each control primer specific for human TFF1 (pS2) promoter. FOR: CCG GCC ATC TCT CAC TAT GAA; REV: CCT TCC CGC CAG GGT AAA TAC
Immunogen	The ERα antibody is made against aa 120-170 of bovine estrogen α.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Estrogen Receptor α

MilliporeSigma™ Upstate™ HDAC1α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for one year from date of shipment
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Supernatant
Gene Accession No.	Q13547
Isotype	IgG1
Includes	This ChIPAb+ HDAC1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	HDAC1α
Regulatory Status	RUO
Gene Symbols	HD1, HDAC1, RPD3L1
Gene ID (Entrez)	NP_032254
Formulation	Anti-HDAC1 (mouse monoclonal IgG1, supernatant). One vial containing 40μL of culture supernatant with 0.05% sodium azide. Negative ChIP Control Supernatant. One vial containing 40μL of mouse IgG containing supernatant with 0.05% sodium azide. ChIP Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. P21 Forward: CCC ACA GCA GAG GAG AAA GAA; P21 Reverse: CTG GAA ATC TCT GCC CAG ACA
Immunogen	The HDAC1 antibody is made against a peptide corresponding to amino acids 467-482 of mouse HDAC1.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	HDAC1

Human Mesenchymal Stem Cell Lineage Antibody Cocktail w/Isotype Control, BD

Pricing & Availability

MilliporeSigma™ Upstate™ FOXA1, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Immunocytochemistry,Western Blot
Gene Accession No.	P55317
Includes	This ChIPAb+ FOXA1 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	FOXA1
Regulatory Status	RUO
Gene Symbols	FOXA1, HNF-3-alpha, HNF-3A, HNF3A, TCF3A, MGC33105
Purification Method	Affinity Purified
Gene ID (Entrez)	NP_004487
Formulation	Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50μL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Concentration: 0.35mg/mL. Normal Rabbit IgG. One vial containing 125μg Rabbit IgG in 125μL storage buffer containing 0.05% sodium azide. ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75μL of 5μM of each primer specific for Mouse Hnf4α enhancer. FOR: TTC CAG CTG CCT TTA TCT CCC TGT; REV: TCT CCA CAC ATG TCC AGC AGC CT
Immunogen	Recombinant protein corresponding to the entire protein of human FOXA1
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	This antibody recognizes HNF-3A/FOXA1.

BD FoxP3 Staining Kit

High performance reagent kit for detection of human FoxP3 positive regulatory T cells

Pricing & Availability

MilliporeSigma™ RIPAb+™ PUM2 RIP Validated Antibody and Primer Set

Detect RIPAb+ PUM2 using this RIPAb+ PUM2 Antibody validated for use in ICC, IHC(P), Western Blotting, RNA Binding Protein Immunoprecipitation (RIP).

Pricing & Availability

MilliporeSigma™ Upstate™ Acetyl-Histone H3 (Lys27)α, Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C for up to 12 months; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	Q66I33
Isotype	IgG
Includes	Acetyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Ac.
Antigen	Acetyl-Histone H3 (Lys27)α
Regulatory Status	RUO
Gene ID (Entrez)	NM_003493
Formulation	Anti-acetyl-Histone H3 (Lys27) (mouse monoclonal IgG1, Clone CMA309). One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, RPL10 Promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG
Immunogen	The acetyl-histone H3 (Lys27) purified antibody is made against a synthetic peptide (acetylated at Lys27) corresponding to amino acids 19-37 of histone H3.
Classification	Monoclonal
Primary or Secondary	Primary
Test Specificity	Recognizes histone H3, Mr 17kDa, acetylated at Lys27.

MilliporeSigma™ Upstate™ Nanog Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Mouse
Applications	ChIP Assay,Immunocytochemistry,Western Blot
Form	Purified
Gene Accession No.	Q9H9S0
Includes	Use ChIPAb+ Nanog ChIP Validated mouse Monoclonal for the detection of Nanog.
Antigen	Nanog
Regulatory Status	RUO
Gene Symbols	NANOG
Purification Method	Protein G purified
Gene ID (Entrez)	NP_079141
Formulation	Anti-Nanog (Mouse Monoclonal). One vial containing 110μL (0.7 mg/mL) purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4),150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, miR-302. One vial containing 75μL of 5μM of each primer specific for human miR-302 cluster promoter region (chr4:113570181-113570261, hg19 build). FOR: CCC GTG GAA GCA ATC TAT TTA TTT; REV: TGT GTT TCT ATC TGG AGG AAC TCT GT
Immunogen	GST-tagged recombinant protein corresponding to human NANOG.
Classification	Monoclonal
Primary or Secondary	Primary

T Cell Exhaustion Marker (Flow-Directly Conjugated) Antibody Pack, R&D Systems™

A Novus product, part of R&D Systems. Antibody Pack

Pricing & Availability

MilliporeSigma™ Upstate™ Acetyl-Histone H4, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	−20°C from date of receipt
Host Species	Rabbit
Applications	Multiplex,Functional Assay,Immunoassay,Western Blot
Form	Serum
Gene Accession No.	P62805
Includes	This ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	Acetyl-Histone H4
Regulatory Status	RUO
Gene ID (Entrez)	NP_778224
Formulation	Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for for human GAPDH.
Immunogen	The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes acetylated histone H4 of approximately 10kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins.

BD Biotin Human CD4 T Lymphocyte Enrichment Set - DM

For the negative selection of CD4 T lymphocytes from peripheral blood

Pricing & Availability

MilliporeSigma™ RIPAb+™ hnRNP U RIP Validated Antibody and Primer Set

This RIPAb+ hnRNP U -RIP Validated Antibody and Primer Set conveniently includes the hnRNP U antibody and the specific control PCR primers.

Pricing & Availability

MilliporeSigma™ Upstate™ EED, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Host Species	Rabbit
Applications	ChIP Assay,Western Blot
Form	Purified
Gene Accession No.	O75530
Includes	This ChIPAb+ EED -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
Antigen	EED
Regulatory Status	RUO
Gene Symbols	EED, WAIT-1
Gene ID (Entrez)	NP_003788
Formulation	Anti-EED (Rabbit polyclonal IgG). One vial containing 50μg of affinity purified antibody in 50μL of 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl, containing 0.05% azide. Normal rabbit IgG. 1 vial containing 125μg of purified rabbit IgG in 125μL of storage buffer containing 0.1% sodium azide. ChIP Primers, HoxA2 upstream. One vial containing 75μL of 5μM of each primer specific for the promoter region of human HoxA2. FOR: AGG AAA GAT TTT GGT TGG GAA G; REV: AAA AAG AGG GAA AGG GAC AGA C
Immunogen	A synthetic peptide corresponding to a.a. 429-441 of human EED,conjugated to KLH.
Classification	Polyclonal
Primary or Secondary	Primary
Test Specificity	Recognizes EED, Mr 62 to 70kDa.

Dog Compensation Set, Clone: LSM 1.140, LSM 12.125, LSM 8.358, CTL 2.58 , BD

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